Reporter

Part:BBa_K362047:Design

Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama   Group: iGEM10_KIT-Kyoto   (2010-10-25)


yaiA　promoter - RBS - YFP - Term - Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 351
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 351
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 351
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part has the promoter ,yaiA promoter controlled by OxyR protein, and YFP , double terminator.


Source

yaiA promoter from E.coli genome

References